SPEAKER AND GENDER IDENTIFICATION USING BIOACOUSTIC DATA SETS

Acoustic analysis of animal vocalizations has been widely used to identify the presence of individual species, classify vocalizations, identify individuals, and determine gender. In this work automatic identification of speaker and gender of mice from ultrasonic vocalizations and speaker identification of meerkats from their Close calls is investigated. Feature extraction was implemented using Greenwood Function Cepstral Coefficients (GFCC), designed exclusively for extracting features from animal vocalizations. Mice ultrasonic vocalizations were analyzed using Gaussian Mixture Models (GMM) which yielded an accuracy of 78.3% for speaker identification and 93.2% for gender identification. Meerkat speaker identification with Close calls was implemented using Gaussian Mixture Models (GMM) and Hidden Markov Models (HMM), with an accuracy of 90.8% and 94.4% respectively. The results obtained shows these methods indicate the presence of gender and identity information in vocalizations and support the possibility of robust gender identification and individual identification using bioacoustic data sets

Positive selection in the mitochondrial protein coding genes of teleost regional endotherms: Evidence for adaptive evolution

Mitochondrial oxidative phosphorylation genes play critical role in energy metabolism, aerobic potential and thermogenesis. These genes were thought to evolve neutrally, however increasing evidence suggests that mitogenome is susceptible to selection and adaptive variation. Organisms that have encountered selective forces to improve their metabolic potential or adapt to cooler environment can be suitable candidates to study the pattern and impact of selection on mitochondrial genome. Tunas, billfishes, butterfly mackerel and opah are the only teleost fishes to exhibit regional endothermy. They might have experienced strong selective forces to enhance their metabolic potential making them a suitable candidate group to search for positive selection. Mitochondrial protein coding genes of 16 regionally endothermic teleosts retrieved from NCBI GenBank were used to examine the pattern of selection using different ω-based approaches implemented in DATAMONKEY and TreeSAAP to analyze the changes in physicochemical properties of the amino acids. We found evidence for positive selection in different mitochondrial protein subunits across several branches of the phylogeny. Changes found in the subunits ND5 and ND6 might have modified the proton pumping efficiency and assembly of complex I respectively and the substitutions found in the subunit ATP6 might have an impact on the rotor performance of the complex V. Further studies on assessment of metabolic consequences of OXPHOS substitutions are essential to understand the importance of these substitutions on the performance of the fishe

Comparative mitogenomics and phylogenetics of the family Carangidae with special emphasis on the mitogenome of the Indian Scad Decapterus russelli

Carangids are abundant and commercially important marine fish that contribute to a significant portion of the fisheries in many parts of the world. In the present study, we characterized the complete mitogenome of the Indian scad, Decapterus russelli and performed a comprehensive comparative mitogenomic analysis of the family Carangidae. The comparative mitogenomics provided valuable insights into the structure, variability, and features of the coding and non-coding regions that evolved across species over millions of years. The structural features of tRNAs revealed changes in the frequency of mismatched and wobble base pairs, which is reflected in the base composition of H and L strands. The highly conserved sequence motif of the mTERF binding site in carangids over the ~ 400 MYA of their divergence demonstrated the functional importance of these sites. The control region of carangids was characterized by the presence of discontinuous repeat units with a high rate of sequence divergence in the form of base substitutions, insertions, and deletions. The maintenance of secondary structures in the control region independent of the rapid evolution of primary structure suggested the effect of selective constraints on their maintenance. Maximum likelihood (ML) and Bayesian inference (BI) phylogeny revealed a similar topology consistent with previous taxonomic studies. The extant carangids diverged through the evolutionary events experienced during the Cretaceous, Paleogene, and Neogene periods

Genetic analyses reveal panmixia in Indian waters and population subdivision across Indian Ocean and Indo-Malay Archipelago for Decapterus russelli

Abstract The Indian Scad, Decapterus russelli is an important pelagic carangid widely distributed throughout the Indian Ocean and the Indo-West Pacific. Despite being widely distributed in the Indian Ocean, the information regarding genetic structuring and diversity has been lacking compared to its Indo Malay Archipelago counterparts. The present study was conducted to investigate the genetic stock structure of D. russelli based on mitochondrial (Cyt b) and nuclear (DrAldoB1) markers along Indian waters. The results indicated the presence of a single panmictic stock across the Indian Ocean region. High haplotype diversity associated with low nucleotide diversity suggested a population bottleneck followed by rapid population growth. Phylogenetic analysis revealed the absence of geographical clustering of lineages with the most common haplotype distributed globally. The pelagic life style, migratory capabilities, and larval dispersal may be the contributing factors to the observed spatial homogeneity of D. russelli. However, significant genetic differentiation was observed between the populations from Indian Ocean and Indo-Malay Archipelago. Hierarchical molecular variance analysis (AMOVA), pairwise FST comparisons and SAMOVA showed existence of two distinct genetic stocks of D. russelli in the Indian Ocean and IMA. The observed interpopulation genetic variation was high. A plausible explanation for the genetic differentiation observed between the Indo-Malay Archipelago and the Indian Ocean regions suggest the influence of historic isolation, ocean surface currents and biotic and abiotic features of the ocean. Also, there was a significant relationship between genetic distance and geographical distance between population pairs in a manner consistent with isolation-by-distance. These resulted in the evolution of a phylogeographic break for this species between these regions. The findings of these results suggest that D. russelli from the Indian Ocean shall be managed in its entire area of distribution as a single stock. Further, the Indian Ocean and Indo-Malayan stocks can be managed separately